| PULID | Characterization Method(s) | Substrate | Organism | Publication | Publish Date | Type | Num Genes | Num CAZymes | CazyFamily |
|---|---|---|---|---|---|---|---|---|---|
| PUL0033 | RT-PCR, yeast two hybrid assay, Southern Blot | trehalose | Spiroplasma citri | 12949193 Glucose and trehalose PTS permeases of Spiroplasma citri probably share a single IIA domain, enabling the spiroplasma to adapt quickly to carbohydrate changes in its environment. Microbiology (Reading). 2003 Sep;149(Pt 9):2687-2696. doi: 10.1099/mic.0.26336-0. |
2003 Sep | degradation | 6 | 1 | GH13_29, GH13 |
| PUL0036 | Southern Blot, transposon mutagenesis | emulsan | Acinetobacter venetianus | 11429470 Analysis of the wee gene cluster responsible for the biosynthesis of the polymeric bioemulsifier from the oil-degrading strain Acinetobacter lwoffii RAG-1. Microbiology (Reading). 2001 Jul;147(Pt 7):1937-1946. doi: 10.1099/00221287-147-7-1937. |
2001 Jul | biosynthesis | 22 | 3 | PL12, PL0, GT4 |
| PUL0038 | enzyme activity assay, Southern Blot | melibiose, galactose | Thermus brockianus | 10741834 The structure of the alpha-galactosidase gene loci in Thermus brockianus ITI360 and Thermus thermophilus TH125. Extremophiles. 2000 Feb;4(1):23-33. doi: 10.1007/s007920050004. |
2000 Feb | degradation | 8 | 2 | GH42, GH36 |
| PUL0039 | enzyme activity assay, Southern Blot | melibiose, galactose | Thermus thermophilus | 10741834 The structure of the alpha-galactosidase gene loci in Thermus brockianus ITI360 and Thermus thermophilus TH125. Extremophiles. 2000 Feb;4(1):23-33. doi: 10.1007/s007920050004. |
2000 Feb | degradation | 3 | 1 | GH36 |
| PUL0041 | Southern Blot, enzyme activity assay | cellobiose | Klebsiella oxytoca | 9023916 Cloning of cellobiose phosphoenolpyruvate-dependent phosphotransferase genes: functional expression in recombinant Escherichia coli and identification of a putative binding region for disaccharides. Appl Environ Microbiol. 1997 Feb;63(2):355-63. doi: 10.1128/aem.63.2.355-363.1997. |
1997 Feb | degradation | 3 | 1 | GH1 |
| PUL0088 | Southern Blot | raffinose, melibiose, isomaltotriose | Streptococcus mutans | 8764489 The multiple-sugar metabolism (msm) gene cluster of Streptococcus mutans is transcribed as a single operon. FEMS Microbiol Lett. 1996 Jul 1;140(2-3):261-4. doi: 10.1016/0378-1097(96)00191-7. |
1996 Jul 1 | degradation | 8 | 3 | GH36, GH13_18, GH13 |
| PUL0100 | transposon mutagenesis, growth assay | chitobiose | Escherichia coli | 9405618 Wild-type Escherichia coli grows on the chitin disaccharide, N,N'-diacetylchitobiose, by expressing the cel operon. Proc Natl Acad Sci U S A. 1997 Dec 23;94(26):14367-71. doi: 10.1073/pnas.94.26.14367. |
1997 Dec 23 | degradation | 6 | 1 | GH4 |
| PUL0321 | enzyme activity assay, transposon mutagenesis | beta-glucoside | Escherichia coli | 19233952 Characterization of a beta-glucoside operon (bgc) prevalent in septicemic and uropathogenic Escherichia coli strains. Appl Environ Microbiol. 2009 Apr;75(8):2284-93. doi: 10.1128/AEM.02621-08. Epub 2009 Feb 20. |
2009 Apr | degradation | 6 | 1 | GH1 |
| PUL0428 | transposon mutagenesis, growth assay | exopolysaccharide | Staphylococcus epidermidis | 8809760 Molecular basis of intercellular adhesion in the biofilm-forming Staphylococcus epidermidis. Mol Microbiol. 1996 Jun;20(5):1083-91. doi: 10.1111/j.1365-2958.1996.tb02548.x. |
1996 Jun | biosynthesis | 5 | 1 | CE4 |
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